prevalence of the caga-positive helicobacter pylori strains isolated from gastric biopsy specimens in shahrekord
نویسندگان
چکیده
abstract: introduction & objective: helicobacter pylori are among the important pathogens responsible for chronic gastritis, peptic ulcer and gastric cancer. the present study aimed to comparatively evaluate pcr and rut methods for detection of h. pylori and determination of the prevalence of the cagapositive helicobacter pylori strains in shahrekord. materials & methods: this is a molecular epidemiology study conducted in 1385 on biopsy samples collected from 120 patients with dyspeptic symptoms who were referred to endoscopy department of hajar hospital of shahrekord. in order to detect h. pylori, rut method was used at first and then dna was directly extracted from biopsy specimens. pcr-amplification was preformed for the urec and then for caga gene. results: the h. pylori infection was found in 74 (61.66%) of the patient by rut method. in parallel, urec pcr detected h. pylori in 103 (85.83%) of patients. all rut-positive patients were found to be urec-pcr positive, too. the caga-positive h. pylori strains were found in 83.5 percent of isolated strains. conclusion: these findings indicate that urec pcr is more sensitive than rut for diagnosis of h. pylori infection. in addition, the high prevalence of the caga-positive h. pylori strains is present in shahrekord.
منابع مشابه
PCR and RFLP Analysis for Identification and Typing of Helicobacter pylori Strains from Gastric Biopsy Specimens
متن کامل
pcr and rflp analysis for identification and typing of helicobacter pylori strains from gastric biopsy specimens
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متن کاملPCR and RFLP Analysis for Identification and Typing of Helicobacter pylori Strains from Gastric Biopsy Specimens
Helicobacter pylori is the causative agent of chronic gastritis and peptic ulcer diseases and is also a risk factor for gastric cancer. Since culture of Helicobacter pylori is relatively insensitive and cumbersome, PCR-based molecular detection and typing of this organism is gaining importance for strain differentiation. The aim of present study was to apply molecular methods for detection and ...
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BACKGROUND The cagA gene is a marker for the presence of the cag pathogenicity island, and the presence of cagA positive strains of Helicobacter pylori can identify individuals with a higher risk of developing gastrointestinal diseases. AIMS To study the interaction between H. pylori cagA(+) and cagA(-) strains and the gastric mucosa. METHODS Patients with H. pylori associated gastritis and...
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عنوان ژورنال:
ارمغان دانشجلد ۱۲، شماره ۱، صفحات ۲۹-۳۸
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